PVP-augmented LISP and DISP AutoAnalyzer haemagglutination channels.

نویسندگان

  • J Crockford
  • B P Moore
چکیده

A low-ionic strength-Polybrene (LISP) AutoAnalyzer haemagglutination channel (Lalezari, 1968; Perrault and Hogman, 1971) fails to detect a proportion of antibodies, particularly anti-KI, anti-Lewis, and anti-P1, which can be demonstrated by manual techniques (Moore and Perrrault, 1977). Moore et al. (1977) found that when dialysis was used instead of dilution to achieve a low ionic-strength reaction environment (dialysed serum-Polybrene or DISP), a greater proportion of anti-KI and anti-Lewis antibodies was detected. We have found that the addition of PVP (K-90) to cell suspension medium and Polybrene solution improves the effectiveness of both LISP and DISP channels for antibody detection and identification.

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عنوان ژورنال:
  • Journal of clinical pathology

دوره 32 8  شماره 

صفحات  -

تاریخ انتشار 1979